4L55

X-ray structure of the adduct between bovine pancreatic ribonuclease and AziRu

Summary for 4L55

• Entry DOI: 10.2210/pdb4l55/pdb
• Related: 1JVT 1JVU 1JVV 4J1A 4J1B 4LFP 4LFX 4LGK
• Descriptor: Ribonuclease pancreatic, RUTHENIUM ION (3 entities in total)
• Functional Keywords: hydrolase, rna binding
• Biological source: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• Cellular location: Secreted: P61823
• Total number of polymer chains: 2
• Total formula weight: 27618.79

Authors

Vergara, A.,Russo Krauss, I.,Merlino, A. (deposition date: 2013-06-10, release date: 2014-01-29, Last modification date: 2024-10-09)

Primary citation

Vergara, A.,Russo Krauss, I.,Montesarchio, D.,Paduano, L.,Merlino, A.
Investigating the ruthenium metalation of proteins: X-ray structure and Raman microspectroscopy of the complex between RNase A and AziRu.
Inorg.Chem., 52:10714-10716, 2013

PubMed Abstract: A Raman-assisted crystallographic study on the adduct between AziRu, a Ru(III) complex with high antiproliferative activity, and RNase A is presented. The protein structure is not perturbed significantly by the Ru label. The metal coordinates to ND atoms of His105 or of His119 imidazole rings, losing all of its original ligands but retaining octahedral, although distorted, coordination geometry. The AziRu binding inactivates the enzyme, suggesting that its antitumor action can be exerted by a mechanism of competitive inhibition.

PubMed: 24093479
DOI: 10.1021/ic401494v

Experimental method

X-RAY DIFFRACTION (1.65 Å)