4L55
X-ray structure of the adduct between bovine pancreatic ribonuclease and AziRu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.104, 32.532, 72.484 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 22.140 - 1.650 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.24400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.140 |
| High resolution limit [Å] | 1.650 |
| Rmerge | 0.099 |
| Number of reflections | 27646 |
| Completeness [%] | 96.4 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 20% PEG 4000, sodium citrate, pH 5.00, VAPOR DIFFUSION, HANGING DROP at 298K soaked in a satured solution of AziRu |
