4DM6

Crystal structure of RARb LBD homodimer in complex with TTNPB

4DM6 の概要

• エントリーDOI: 10.2210/pdb4dm6/pdb
• 関連するPDBエントリー: 1XAP 4DM8 4DMA
• 分子名称: Retinoic acid receptor beta, Nuclear receptor coactivator 1, 4-[(1E)-2-(5,5,8,8-TETRAMETHYL-5,6,7,8-TETRAHYDRONAPHTHALEN-2-YL)PROP-1-ENYL]BENZOIC ACID, ... (4 entities in total)
• 機能のキーワード: transcription, nuclear receptor, retinoic acid, alpha helical sandwich, transcription regulator, retinoic acid binding, nucleus - cytoplasm, transcription-protein binding complex, transcription/protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Isoform Beta-1: Nucleus. Isoform Beta-2: Nucleus. Isoform Beta-4: Cytoplasm: P10826; Nucleus (By similarity): Q15788
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 66667.20

構造登録者

Osz, J.,Brelivet, Y.,Peluso-Iltis, C.,Cura, V.,Eiler, S.,Ruff, M.,Bourguet, W.,Rochel, N.,Moras, D. (登録日: 2012-02-07, 公開日: 2012-03-07, 最終更新日: 2024-02-28)

主引用文献

Osz, J.,Brelivet, Y.,Peluso-Iltis, C.,Cura, V.,Eiler, S.,Ruff, M.,Bourguet, W.,Rochel, N.,Moras, D.
Structural basis for a molecular allosteric control mechanism of cofactor binding to nuclear receptors.
Proc.Natl.Acad.Sci.USA, 109:E588-E594, 2012

PubMed Abstract: Transcription regulation by steroid hormones, vitamin derivatives, and metabolites is mediated by nuclear receptors (NRs), which play an important role in ligand-dependent gene expression and human health. NRs function as homodimers or heterodimers and are involved in a combinatorial, coordinated and sequentially orchestrated exchange between coregulators (corepressors, coactivators). The architecture of DNA-bound functional dimers positions the coregulators proteins. We previously demonstrated that retinoic acid (RAR-RXR) and vitamin D3 receptors (VDR-RXR) heterodimers recruit only one coactivator molecule asymmetrically without steric hindrance for the binding of a second cofactor. We now address the problem of homodimers for which the presence of two identical targets enhances the functional importance of the mode of binding. Using structural and biophysical methods and RAR as a model, we could dissect the molecular mechanism of coactivator recruitment to homodimers. Our study reveals an allosteric mechanism whereby binding of a coactivator promotes formation of nonsymmetrical RAR homodimers with a 21 stoichiometry. Ligand conformation and the cofactor binding site of the unbound receptor are affected through the dimer interface. A similar control mechanism is observed with estrogen receptor (ER) thus validating the negative cooperativity model for an established functional homodimer. Correlation with published data on other NRs confirms the general character of this regulatory pathway.

PubMed: 22355136
DOI: 10.1073/pnas.1118192109

実験手法

X-RAY DIFFRACTION (1.9 Å)