4D8D

Crystal structure of HIV-1 NEF Fyn-SH3 R96W variant

4D8D の概要

• エントリーDOI: 10.2210/pdb4d8d/pdb
• 関連するPDBエントリー: 3H0F 3H0H 3H0I
• 分子名称: Tyrosine-protein kinase Fyn, Protein Nef, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: signaling molecules, transferase-protein binding complex, transferase/protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm: P06241; Host cell membrane ; Lipid-anchor ; Cytoplasmic side : P03406
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48533.96

構造登録者

Arold, S.T.,Hoh, F.,Dumas, C. (登録日: 2012-01-10, 公開日: 2013-01-16, 最終更新日: 2023-09-13)

主引用文献

Aldehaiman, A.,Momin, A.A.,Restouin, A.,Wang, L.,Shi, X.,Aljedani, S.,Opi, S.,Lugari, A.,Shahul Hameed, U.F.,Ponchon, L.,Morelli, X.,Huang, M.,Dumas, C.,Collette, Y.,Arold, S.T.
Synergy and allostery in ligand binding by HIV-1 Nef.
Biochem.J., 478:1525-1545, 2021

PubMed Abstract: The Nef protein of human and simian immunodeficiency viruses boosts viral pathogenicity through its interactions with host cell proteins. By combining the polyvalency of its large unstructured regions with the binding selectivity and strength of its folded core domain, Nef can associate with many different host cell proteins, thereby disrupting their functions. For example, the combination of a linear proline-rich motif and hydrophobic core domain surface allows Nef to bind tightly and specifically to SH3 domains of Src family kinases. We investigated whether the interplay between Nef's flexible regions and its core domain could allosterically influence ligand selection. We found that the flexible regions can associate with the core domain in different ways, producing distinct conformational states that alter the way in which Nef selects for SH3 domains and exposes some of its binding motifs. The ensuing crosstalk between ligands might promote functionally coherent Nef-bound protein ensembles by synergizing certain subsets of ligands while excluding others. We also combined proteomic and bioinformatics analyses to identify human proteins that select SH3 domains in the same way as Nef. We found that only 3% of clones from a whole-human fetal library displayed Nef-like SH3 selectivity. However, in most cases, this selectivity appears to be achieved by a canonical linear interaction rather than by a Nef-like 'tertiary' interaction. Our analysis supports the contention that Nef's mode of hijacking SH3 domains is a virus-specific adaptation with no or very few cellular counterparts. Thus, the Nef tertiary binding surface is a promising virus-specific drug target.

PubMed: 33787846
DOI: 10.1042/BCJ20201002

実験手法

X-RAY DIFFRACTION (2.5201 Å)