4D8D
Crystal structure of HIV-1 NEF Fyn-SH3 R96W variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-04-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.98064 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 108.010, 108.010, 224.961 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.513 - 2.520 |
R-factor | 0.2135 |
Rwork | 0.212 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1avz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.977 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.510 |
High resolution limit [Å] | 2.520 |
Number of reflections | 26791 |
Completeness [%] | 99.1 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 0.3 M sodium potassium tartrate, 0.5 M bicine buffer , 1cmc D-octylglucopyranoside, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |