4BDV

CRYSTAL STRUCTURE OF A TRUNCATED B-DOMAIN HUMAN FACTOR VIII

Summary for 4BDV

• Entry DOI: 10.2210/pdb4bdv/pdb
• Related: 1CFG 1D7P 1IQD
• Descriptor: FACTOR VIIIA HEAVY CHAIN, 92 KDA ISOFORM, B DOMAIN, FACTOR VIIIA LIGHT CHAIN, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• Functional Keywords: blood clotting, blood coagulation, metal binding
• Biological source: HOMO SAPIENS (HUMAN); More
• Total number of polymer chains: 2
• Total formula weight: 167977.58

Authors

Svensson, L.A.,Thim, L.,Olsen, O.H.,Nicolaisen, E.M. (deposition date: 2012-10-08, release date: 2013-05-15, Last modification date: 2024-10-23)

Primary citation

Svensson, L.A.,Thim, L.,Olsen, O.H.,Nicolaisen, E.M.
Evaluation of the Metal Binding Sites in a Recombinant Coagulation Factor Viii Identifies Two Sites with Unique Metal Binding Properties.
Biol.Chem., 394:761-, 2013

PubMed Abstract: Coagulation factor VIII is a glycosylated, non-covalent heterodimer consisting of a heavy chain (A1-A2-B domains) and a light chain (A3-C1-C2 domains). The association of the chains, and the stability and function of the dimer depend on the presence of metal ions. We applied X-ray fluorescence, X-ray crystallographic structure determination with anomalous signals at different wavelengths, and colorimetric measurements to evaluate the metal binding sites in a recombinant factor VIII molecule, turoctocog alfa. We identified a metal binding site in domain A3 dominated by Cu(+) binding and a site in domain A1 dominated by Zn(2+) binding.

PubMed: 23435097
DOI: 10.1515/HSZ-2012-0298

Experimental method

X-RAY DIFFRACTION (3.98 Å)