4AS8

X-ray structure of the cyan fluorescent protein Cerulean cryoprotected with ethylene glycol

4AS8 の概要

• エントリーDOI: 10.2210/pdb4as8/pdb
• 関連するPDBエントリー: 1B9C 1BFP 1C4F 1CV7 1EMA 1EMB 1EMC 1EME 1EMF 1EMG 1EMK 1EML 1EMM 1F09 1F0B 1GFL 1H6R 1HCJ 1HUY 1JBY 1JBZ 1JC0 1JC1 1KP5 1KYP 1KYR 1KYS 1MYW 1Q4A 1Q4B 1Q4C 1Q4D 1Q4E 1Q73 1QXT 1QY3 1QYF 1QYO 1QYQ 1RM9 1RMM 1RMO 1RMP 1RRX 1W7S 1W7T 1W7U 1YFP 1YHG 1YHH 1YHI 1YJ2 1YJF 1Z1P 1Z1Q 2AH8 2AHA 2B3P 2B3Q 2EMD 2EMN 2EMO 2FWQ 2FZU 2WSN 2WSO 2WUR 2Y0G 2YDZ 2YE0 2YE1 2YFP 3ZTF 4ANJ 4AR7 4B5Y
• 分子名称: GREEN FLUORESCENT PROTEIN, 1,2-ETHANEDIOL, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: fluorescent protein, fret
• 由来する生物種: AEQUOREA VICTORIA (JELLYFISH)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28325.17

構造登録者

von Stetten, D.,Batot, G.,Noirclerc-Savoye, M.,Royant, A. (登録日: 2012-04-29, 公開日: 2012-10-31, 最終更新日: 2024-11-13)

主引用文献

von Stetten, D.,Batot, G.,Noirclerc-Savoye, M.,Royant, A.
Alteration of Fluorescent Protein Spectroscopic Properties Upon Cryoprotection
Acta Crystallogr.,Sect.D, 68:1578-, 2012

PubMed Abstract: Cryoprotection of a protein crystal by addition of small-molecule compounds may sometimes affect the structure of its active site. The spectroscopic and structural effects of the two cryoprotectants glycerol and ethylene glycol on the cyan fluorescent protein Cerulean were investigated. While glycerol had almost no noticeable effect, ethylene glycol was shown to induce a systematic red shift of the UV-vis absorption and fluorescence emission spectra. Additionally, ethylene glycol molecules were shown to enter the core of the protein, with one of them binding in close vicinity to the chromophore, which provides a sound explanation for the observed spectroscopic changes. These results highlight the need to systematically record spectroscopic data on crystals of light-absorbing proteins and reinforce the notion that fluorescent proteins must not been seen as rigid structures.

PubMed: 23090407
DOI: 10.1107/S0907444912037900

実験手法

X-RAY DIFFRACTION (1.02 Å)