2FWQ

Reduced enolate chromophore intermediate for Y66H GFP variant

Summary for 2FWQ

DescriptorGreen fluorescent protein, MAGNESIUM ION (3 entities in total)
Functional Keywordsbeta barrel, chromophore, biosynthesis, intermediate, enolate, dithionite, luminescent protein
Biological sourceAequorea victoria
Total number of polymer chains1
Total molecular weight26753.32
Authors
Barondeau, D.P.,Tainer, J.A.,Getzoff, E.D. (deposition date: 2006-02-02, release date: 2006-03-14, Last modification date: 2017-10-11)
Primary citation
Barondeau, D.P.,Tainer, J.A.,Getzoff, E.D.
Structural evidence for an enolate intermediate in GFP fluorophore biosynthesis.
J.Am.Chem.Soc., 128:3166-3168, 2006
PubMed: 16522096 (PDB entries with the same primary citation)
DOI: 10.1021/ja0552693
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.4 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.198202.5%3.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2fwq
no rotation
Molmil generated image of 2fwq
rotated about x axis by 90°
Molmil generated image of 2fwq
rotated about y axis by 90°
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