1YFP

STRUCTURE OF YELLOW-EMISSION VARIANT OF GFP

Summary for 1YFP

Entry DOI	10.2210/pdb1yfp/pdb
Descriptor	YELLOW FLUORESCENT PROTEIN (2 entities in total)
Functional Keywords	luminescence, green fluorescent protein, yellow-emission variant, bioluminescence, photoactive protein, fluorescent tag
Biological source	Aequorea victoria
Total number of polymer chains	2
Total formula weight	51419.99
Authors	Wachter, R.M.,Elsliger, M.-A.,Kallio, K.,Hanson, G.T.,Remington, S.J. (deposition date: 1998-08-28, release date: 1998-10-28, Last modification date: 2024-10-16)
Primary citation	Wachter, R.M.,Elsliger, M.A.,Kallio, K.,Hanson, G.T.,Remington, S.J. Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure, 6:1267-1277, 1998 Cited by PubMed Abstract: Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. PubMed: 9782051 DOI: 10.1016/S0969-2126(98)00127-0 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation