4AS8
X-ray structure of the cyan fluorescent protein Cerulean cryoprotected with ethylene glycol
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | GREEN FLUORESCENT PROTEIN | polymer | 245 | 27618.1 | 1 | UniProt (P42212) Pfam (PF01353) In PDB | AEQUOREA VICTORIA (JELLYFISH) | CYAN FLUORESCENT PROTEIN CERULEAN |
2 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
3 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
4 | water | water | 18.0 | 256 | Chemie (HOH) |
Sequence modifications
A: 2 - 238 (UniProt: P42212)
PDB | External Database | Details |
---|---|---|
Met 0 | - | expression tag |
Val 1 | - | expression tag |
His 239 | - | expression tag |
His 240 | - | expression tag |
His 241 | - | expression tag |
His 242 | - | expression tag |
His 243 | - | expression tag |
His 244 | - | expression tag |
Leu 64 | Phe 64 | engineered mutation |
Crf 66 | Ser 65 | chromophore |
Crf 66 | Tyr 66 | chromophore |
Crf 66 | Gly 67 | chromophore |
Ala 72 | Ser 72 | engineered mutation |
Ala 145 | Tyr 145 | engineered mutation |
Ile 146 | Asn 146 | engineered mutation |
Asp 148 | His 148 | engineered mutation |
Thr 153 | Met 153 | engineered mutation |
Ala 163 | Val 163 | engineered mutation |
Leu 231 | His 231 | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 27618.1 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 707.1 | |
All* | Total formula weight | 28325.2 |