4AS8

X-ray structure of the cyan fluorescent protein Cerulean cryoprotected with ethylene glycol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GREEN FLUORESCENT PROTEIN	polymer	245	27618.1	1	UniProt (P42212) Pfam (PF01353)	AEQUOREA VICTORIA (JELLYFISH)	CYAN FLUORESCENT PROTEIN CERULEAN
2	B, C, D, E, F... (A)	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
3	M (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	N (A)	water	water		18.0	256	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	expression tag
Val 1	-	expression tag
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag
Leu 64	Phe 64	engineered mutation
Crf 66	Ser 65	chromophore
Crf 66	Tyr 66	chromophore
Crf 66	Gly 67	chromophore
Ala 72	Ser 72	engineered mutation
Ala 145	Tyr 145	engineered mutation
Ile 146	Asn 146	engineered mutation
Asp 148	His 148	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Leu 231	His 231	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27618.1
Non-Polymers*	Number of molecules	12
	Total formula weight	707.1
All*	Total formula weight	28325.2

*Water molecules are not included.