4AAG

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Summary for 4AAG

• Entry DOI: 10.2210/pdb4aag/pdb
• Related: 1AF5 1BP7 1G9Y 1G9Z 1MOW 1N3E 1N3F 1T9I 1T9J 1U0C 1U0D 2VBJ 2VBL 2VBN 2VBO 4AAB 4AAD 4AAE 4AAF
• Descriptor: DNA ENDONUCLEASE I-CREI, 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP *GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3', CALCIUM ION, ... (4 entities in total)
• Functional Keywords: hydrolase-dna complex, gene targeting, protein-dna interaction, homing endonucleases, hydrolase/dna
• Biological source: CHLAMYDOMONAS REINHARDTII; More
• Cellular location: Plastid, chloroplast: P05725
• Total number of polymer chains: 4
• Total formula weight: 49847.99

Authors

Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.,Epinat, J.C.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2011-12-01, release date: 2012-05-02, Last modification date: 2023-12-20)

• Primary citation: Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Charles Epinat, J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.; Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.; Nucleic Acids Res., 40:6936-, 2012; PubMed: 22495931; DOI: 10.1093/NAR/GKS320

Experimental method

X-RAY DIFFRACTION (2.8 Å)