4AAG

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0006314	biological_process	intron homing
A	0009507	cellular_component	chloroplast
A	0016787	molecular_function	hydrolase activity
A	0042802	molecular_function	identical protein binding
A	0046872	molecular_function	metal ion binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0006314	biological_process	intron homing
B	0009507	cellular_component	chloroplast
B	0016787	molecular_function	hydrolase activity
B	0042802	molecular_function	identical protein binding
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA A 1154

Chain	Residue
A	ASP20
A	HOH2007
A	HOH2018
B	GLY19
E	DC514
G	DG615

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site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE CA B 1154

Chain	Residue
B	HOH2015
E	DG515
G	DC614
A	GLY19
B	ASP20
B	HOH2001

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	44
Details	Region: {"description":"Interaction with DNA"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	2
Details	M-CSA 233

Chain	Residue	Details
A	GLY19	metal ligand
A	ASP20	metal ligand

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site_id	MCSA2
Number of Residues	2
Details	M-CSA 233

Chain	Residue	Details
B	GLY19	metal ligand
B	ASP20	metal ligand

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