4AAG

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DNA ENDONUCLEASE I-CREI	polymer	152	17515.1	2	UniProt (P05725) Pfam (PF00961) In PDB	CHLAMYDOMONAS REINHARDTII	23S RRNA INTRON PROTEIN, I-CREI
2	E, G	5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP *GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3'	polymer	24	7368.8	2		SYNTHETIC CONSTRUCT
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4		water	water		18.0	40	Chemie (HOH)

Sequence modifications

A, B: 2 - 153 (UniProt: P05725)

PDB	External Database	Details
Asn 75	Asp 75	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	49767.8
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	49848.0

*Water molecules are not included.