4AAF

Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by TGCA from 5' to 3')

Summary for 4AAF

Related1G9Z 1BP7 2VBL 4AAE 2VBO 1MOW 1U0D 4AAD 1T9I 1T9J 1N3E 4AAB 1AF5 2VBJ 1G9Y 1N3F 2VBN 1U0C 4AAG
DescriptorDNA ENDONUCLEASE I-CREI, 24MER DNA, 1,2-ETHANEDIOL, ... (4 entities in total)
Functional Keywordshydrolase-dna complex, gene targeting, protein-dna interaction, homing endonucleases, hydrolase/dna
Biological sourceCHLAMYDOMONAS REINHARDTII
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Cellular locationPlastid, chloroplast P05725
Total number of polymer chains4
Total molecular weight49829.9
Authors
Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Epinat, J.C.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2011-12-01, release date: 2012-05-02, Last modification date: 2012-08-22)
Primary citation
Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Charles Epinat, J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.
Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40:6936-, 2012
PubMed: 22495931 (PDB entries with the same primary citation)
DOI: 10.1093/NAR/GKS320
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.2341002.5%0MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution