4A32

CRYSTAL STRUCTURE OF LEISHMANIA MAJOR N-MYRISTOYLTRANSFERASE (NMT) WITH BOUND MYRISTOYL-COA AND A PYRAZOLE SULPHONAMIDE LIGAND

4A32 の概要

• エントリーDOI: 10.2210/pdb4a32/pdb
• 関連するPDBエントリー: 2WSA 4A2Z 4A30 4A31 4A33
• 分子名称: GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE, GLYCEROL, 3,5-DICHLORO-3'-[(DIETHYLAMINO)METHYL]-N-(1,3,5-TRIMETHYL-1H-PYRAZOL-4-YL)BIPHENYL-4-SULFONAMIDE, ... (5 entities in total)
• 機能のキーワード: acyltransferase, transferase, drug discovery, leishmaniasis
• 由来する生物種: LEISHMANIA MAJOR
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52078.70

構造登録者

Robinson, D.A.,Brand, S.,Cleghorn, L.A.T.,McElroy, S.P.,Smith, V.C.,Hallyburton, I.,Harrison, J.R.,Norcross, N.R.,Norval, S.,Spinks, D.,Stojanovski, L.,Torrie, L.S.,Frearson, J.A.,Brenk, R.,Fairlamb, A.H.,Ferguson, M.A.J.,Read, K.D.,Wyatt, P.G.,Gilbert, I.H. (登録日: 2011-09-29, 公開日: 2011-12-21, 最終更新日: 2023-12-20)

主引用文献

Brand, S.,Cleghorn, L.A.,McElroy, S.P.,Robinson, D.A.,Smith, V.C.,Hallyburton, I.,Harrison, J.R.,Norcross, N.R.,Spinks, D.,Bayliss, T.,Norval, S.,Stojanovski, L.,Torrie, L.S.,Frearson, J.A.,Brenk, R.,Fairlamb, A.H.,Ferguson, M.A.,Read, K.D.,Wyatt, P.G.,Gilbert, I.H.
Discovery of a novel class of orally active trypanocidal N-myristoyltransferase inhibitors.
J. Med. Chem., 55:140-152, 2012

PubMed Abstract: N-Myristoyltransferase (NMT) represents a promising drug target for human African trypanosomiasis (HAT), which is caused by the parasitic protozoa Trypanosoma brucei. We report the optimization of a high throughput screening hit (1) to give a lead molecule DDD85646 (63), which has potent activity against the enzyme (IC(50) = 2 nM) and T. brucei (EC(50) = 2 nM) in culture. The compound has good oral pharmacokinetics and cures rodent models of peripheral HAT infection. This compound provides an excellent tool for validation of T. brucei NMT as a drug target for HAT as well as a valuable lead for further optimization.

PubMed: 22148754
DOI: 10.1021/jm201091t

実験手法

X-RAY DIFFRACTION (2.2 Å)