3TWU

Crystal structure of ARC4 from human Tankyrase 2 in complex with peptide from human MCL1

Summary for 3TWU

• Entry DOI: 10.2210/pdb3twu/pdb
• Related: 3TWQ 3TWR 3TWS 3TWT 3TWV 3TWW 3TWX
• Descriptor: Tankyrase-2, Induced myeloid leukemia cell differentiation protein Mcl-1 (3 entities in total)
• Functional Keywords: ankyrin repeat, protein-protein interaction, substrate recruitment, poly(adp-ribosyl)ation, signaling protein-peptide complex, signaling protein/peptide
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: Q9H2K2; Membrane; Single-pass membrane protein (Potential): Q07820
• Total number of polymer chains: 2
• Total formula weight: 19714.29

Authors

Guettler, S.,Sicheri, F. (deposition date: 2011-09-22, release date: 2011-12-07, Last modification date: 2011-12-28)

Primary citation

Guettler, S.,Larose, J.,Petsalaki, E.,Gish, G.,Scotter, A.,Pawson, T.,Rottapel, R.,Sicheri, F.
Structural basis and sequence rules for substrate recognition by tankyrase explain the basis for cherubism disease.
Cell(Cambridge,Mass.), 147:1340-1354, 2011

PubMed Abstract: The poly(ADP-ribose)polymerases Tankyrase 1/2 (TNKS/TNKS2) catalyze the covalent linkage of ADP-ribose polymer chains onto target proteins, regulating their ubiquitylation, stability, and function. Dysregulation of substrate recognition by Tankyrases underlies the human disease cherubism. Tankyrases recruit specific motifs (often called RxxPDG "hexapeptides") in their substrates via an N-terminal region of ankyrin repeats. These ankyrin repeats form five domains termed ankyrin repeat clusters (ARCs), each predicted to bind substrate. Here we report crystal structures of a representative ARC of TNKS2 bound to targeting peptides from six substrates. Using a solution-based peptide library screen, we derive a rule-based consensus for Tankyrase substrates common to four functionally conserved ARCs. This 8-residue consensus allows us to rationalize all known Tankyrase substrates and explains the basis for cherubism-causing mutations in the Tankyrase substrate 3BP2. Structural and sequence information allows us to also predict and validate other Tankyrase targets, including Disc1, Striatin, Fat4, RAD54, BCR, and MERIT40.

PubMed: 22153077
DOI: 10.1016/j.cell.2011.10.046

Experimental method

X-RAY DIFFRACTION (1.8 Å)