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3TWQ

Crystal structure of ARC4 from human Tankyrase 2 (apo form)

Summary for 3TWQ
Entry DOI10.2210/pdb3twq/pdb
Related3TWR 3TWS 3TWT 3TWU 3TWV 3TWW 3TWX
DescriptorTankyrase-2, SULFATE ION, GLYCEROL, ... (4 entities in total)
Functional Keywordsankyrin repeat, protein-protein interaction, poly(adp-ribosyl)ation, substrate recruitment, signaling protein
Biological sourceHomo sapiens (human)
Cellular locationCytoplasm: Q9H2K2
Total number of polymer chains2
Total formula weight38365.29
Authors
Guettler, S.,Sicheri, F. (deposition date: 2011-09-22, release date: 2011-12-07, Last modification date: 2024-02-28)
Primary citationGuettler, S.,Larose, J.,Petsalaki, E.,Gish, G.,Scotter, A.,Pawson, T.,Rottapel, R.,Sicheri, F.
Structural basis and sequence rules for substrate recognition by tankyrase explain the basis for cherubism disease.
Cell(Cambridge,Mass.), 147:1340-1354, 2011
Cited by
PubMed Abstract: The poly(ADP-ribose)polymerases Tankyrase 1/2 (TNKS/TNKS2) catalyze the covalent linkage of ADP-ribose polymer chains onto target proteins, regulating their ubiquitylation, stability, and function. Dysregulation of substrate recognition by Tankyrases underlies the human disease cherubism. Tankyrases recruit specific motifs (often called RxxPDG "hexapeptides") in their substrates via an N-terminal region of ankyrin repeats. These ankyrin repeats form five domains termed ankyrin repeat clusters (ARCs), each predicted to bind substrate. Here we report crystal structures of a representative ARC of TNKS2 bound to targeting peptides from six substrates. Using a solution-based peptide library screen, we derive a rule-based consensus for Tankyrase substrates common to four functionally conserved ARCs. This 8-residue consensus allows us to rationalize all known Tankyrase substrates and explains the basis for cherubism-causing mutations in the Tankyrase substrate 3BP2. Structural and sequence information allows us to also predict and validate other Tankyrase targets, including Disc1, Striatin, Fat4, RAD54, BCR, and MERIT40.
PubMed: 22153077
DOI: 10.1016/j.cell.2011.10.046
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.151 Å)
Structure validation

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