3QWQ

Crystal structure of the extracellular domain of the epidermal growth factor receptor in complex with an adnectin

Summary for 3QWQ

• Entry DOI: 10.2210/pdb3qwq/pdb
• Related: 3QWR
• Descriptor: Epidermal growth factor receptor, ADNECTIN, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: cell surface receptor, tyrosine kinase, glycoprotein, adnectin, antitumor, drug, engineered binding protein, antibody mimic, signaling protein, protein binding-signaling protein complex, protein binding/signaling protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 87637.55

Authors

Sheriff, S. (deposition date: 2011-02-28, release date: 2012-02-01, Last modification date: 2024-11-20)

Primary citation

Ramamurthy, V.,Krystek, S.R.,Bush, A.,Wei, A.,Emanuel, S.L.,Das Gupta, R.,Janjua, A.,Cheng, L.,Murdock, M.,Abramczyk, B.,Cohen, D.,Lin, Z.,Morin, P.,Davis, J.H.,Dabritz, M.,McLaughlin, D.C.,Russo, K.A.,Chao, G.,Wright, M.C.,Jenny, V.A.,Engle, L.J.,Furfine, E.,Sheriff, S.
Structures of adnectin/protein complexes reveal an expanded binding footprint.
Structure, 20:259-269, 2012

PubMed Abstract: Adnectins are targeted biologics derived from the tenth type III domain of human fibronectin (¹⁰Fn3), a member of the immunoglobulin superfamily. Target-specific binders are selected from libraries generated by diversifying the three ¹⁰Fn3 loops that are analogous to the complementarity determining regions of antibodies. The crystal structures of two Adnectins were determined, each in complex with its therapeutic target, EGFR or IL-23. Both Adnectins bind different epitopes than those bound by known monoclonal antibodies. Molecular modeling suggests that some of these epitopes might not be accessible to antibodies because of the size and concave shape of the antibody combining site. In addition to interactions from the Adnectin diversified loops, residues from the N terminus and/or the β strands interact with the target proteins in both complexes. Alanine-scanning mutagenesis confirmed the calculated binding energies of these β strand interactions, indicating that these nonloop residues can expand the available binding footprint.

PubMed: 22325775
DOI: 10.1016/j.str.2011.11.016

Experimental method

X-RAY DIFFRACTION (2.75 Å)