3QWQ
Crystal structure of the extracellular domain of the epidermal growth factor receptor in complex with an adnectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-05 |
| Detector | RAYONIX MX-300 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.000, 72.100, 262.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.470 - 2.750 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EGFR FROM PDB ENTRY 1NQL AND ADNECTIN FROM PDB ENTRY 1FNF RESIDUES 1421-1437 1444-1466 1470-1491 1502-1509 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 ((DENZO)) |
| Data scaling software | HKL-2000 ((SCALEPACK)) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.13.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.069 | 0.552 |
| Number of reflections | 34285 | |
| <I/σ(I)> | 23.1 | 3.1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 60% Tacsimate, pH 7.0, vapor diffusion, hanging drop, temperature 293K |
