3OHB

Yeast DNA polymerase eta extending from an 8-oxoG lesion

3OHB の概要

• エントリーDOI: 10.2210/pdb3ohb/pdb
• 関連するPDBエントリー: 1JIH 3MFH 3MFI 3OHA
• 分子名称: DNA polymerase eta, 5'-D(*TP*AP*AP*TP*GP*(8OG)P*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3', 5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3', ... (7 entities in total)
• 機能のキーワード: protein-dna complex, transferase/dna, nucleotidyltransferase, dna-directed dna polymerase, dna repair, dna replication, dna binding, nucleotide binding, metal binding, magnesium binding, nucleus, transferase-dna complex
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• 細胞内の位置: Nucleus : Q04049
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67890.75

構造登録者

Silverstein, T.D.,Jain, R.,Aggarwal, A.K. (登録日: 2010-08-17, 公開日: 2010-12-22, 最終更新日: 2023-09-06)

主引用文献

Silverstein, T.D.,Jain, R.,Johnson, R.E.,Prakash, L.,Prakash, S.,Aggarwal, A.K.
Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase eta.
Structure, 18:1463-1470, 2010

PubMed Abstract: 7,8-dihydro-8-oxoguanine (8-oxoG) adducts are formed frequently by the attack of oxygen-free radicals on DNA. They are among the most mutagenic lesions in cells because of their dual coding potential, where, in addition to normal base-pairing of 8-oxoG(anti) with dCTP, 8-oxoG in the syn conformation can base pair with dATP, causing G to T transversions. We provide here for the first time a structural basis for the error-free replication of 8-oxoG lesions by yeast DNA polymerase η (Polη). We show that the open active site cleft of Polη can accommodate an 8-oxoG lesion in the anti conformation with only minimal changes to the polymerase and the bound DNA: at both the insertion and post-insertion steps of lesion bypass. Importantly, the active site geometry remains the same as in the undamaged complex and provides a basis for the ability of Polη to prevent the mutagenic replication of 8-oxoG lesions in cells.

PubMed: 21070945
DOI: 10.1016/j.str.2010.08.019

実験手法

X-RAY DIFFRACTION (2 Å)