3OHB

Yeast DNA polymerase eta extending from an 8-oxoG lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase eta	polymer	520	58813.4	1	UniProt (Q04049) Pfam (PF00817) Pfam (PF11799) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Radiation-sensitive protein 30
2	T	5'-D(*TP*AP*AP*TP*GP*(8OG)P*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3'	polymer	16	5068.3	1
3	P	5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3'	polymer	11	3205.1	1
4	A	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
5	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7		water	water		18.0	601	Chemie (HOH)

Sequence modifications

A: 1 - 513 (UniProt: Q04049)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ala 140	Lys 140	engineered mutation
Trp 144	Ser 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	67086.8
Non-Polymers*	Number of molecules	6
	Total formula weight	804.0
All*	Total formula weight	67890.7

*Water molecules are not included.