3OHB

Yeast DNA polymerase eta extending from an 8-oxoG lesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	87.958, 228.268, 85.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.540 - 2.000
R-factor	0.1576
Rwork	0.156
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1JIH
RMSD bond length	0.013
RMSD bond angle	1.535
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge		0.061	0.576
Number of reflections	58811
<I/σ(I)>	11.5
Completeness [%]	100.0	99.9	100
Redundancy	7.4	7.1	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	30% (w/v) polyethylene glycol 4000, 0.2 M lithium sulfate, 0.1 M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K