3OHA

Yeast DNA polymerase eta inserting dCTP opposite an 8oxoG lesion

Summary for 3OHA

• Entry DOI: 10.2210/pdb3oha/pdb
• Related: 3MFH 3MFI 3OHB
• Descriptor: DNA polymerase eta, 5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3', 5'-D(P*TP*(8OG)P*GP*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3', ... (7 entities in total)
• Functional Keywords: protein-dna complex, nucleotidyltransferase, dna-directed dna polymerase, dna replication, dna repair, dna binding, nucleotide binding, metal binding, nucleus, transferase-dna complex, transferase/dna
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast)
• Cellular location: Nucleus : Q04049
• Total number of polymer chains: 3
• Total formula weight: 66960.14

Authors

Silverstein, T.D.,Jain, R.,Aggarwal, A.K. (deposition date: 2010-08-17, release date: 2010-12-22, Last modification date: 2023-09-06)

Primary citation

Silverstein, T.D.,Jain, R.,Johnson, R.E.,Prakash, L.,Prakash, S.,Aggarwal, A.K.
Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase eta.
Structure, 18:1463-1470, 2010

PubMed Abstract: 7,8-dihydro-8-oxoguanine (8-oxoG) adducts are formed frequently by the attack of oxygen-free radicals on DNA. They are among the most mutagenic lesions in cells because of their dual coding potential, where, in addition to normal base-pairing of 8-oxoG(anti) with dCTP, 8-oxoG in the syn conformation can base pair with dATP, causing G to T transversions. We provide here for the first time a structural basis for the error-free replication of 8-oxoG lesions by yeast DNA polymerase η (Polη). We show that the open active site cleft of Polη can accommodate an 8-oxoG lesion in the anti conformation with only minimal changes to the polymerase and the bound DNA: at both the insertion and post-insertion steps of lesion bypass. Importantly, the active site geometry remains the same as in the undamaged complex and provides a basis for the ability of Polη to prevent the mutagenic replication of 8-oxoG lesions in cells.

PubMed: 21070945
DOI: 10.1016/j.str.2010.08.019

Experimental method

X-RAY DIFFRACTION (2 Å)