3OHA

Yeast DNA polymerase eta inserting dCTP opposite an 8oxoG lesion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase eta	polymer	520	58813.4	1	UniProt (Q04049) Pfam (PF00817) Pfam (PF11799) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Radiation-sensitive protein 30
2	P	5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3'	polymer	11	3205.1	1
3	T	5'-D(P*TP*(8OG)P*GP*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3'	polymer	13	4137.7	1
4	A	2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE	non-polymer		467.2	1	Chemie (DCP)
5	A	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7		water	water		18.0	705	Chemie (HOH)

Sequence modifications

A: 1 - 513 (UniProt: Q04049)

PDB	External Database	Details
Gly -6	-	expression tag
Pro -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ala 140	Lys 140	engineered mutation
Trp 144	Ser 144	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66156.2
Non-Polymers*	Number of molecules	6
	Total formula weight	804.0
All*	Total formula weight	66960.1

*Water molecules are not included.