3OHA
Yeast DNA polymerase eta inserting dCTP opposite an 8oxoG lesion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.039, 227.635, 86.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.820 - 2.000 |
| R-factor | 0.15928 |
| Rwork | 0.157 |
| R-free | 0.19511 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1JIH |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.032 | 0.613 | |
| Number of reflections | 58857 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6.1 | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30% (w/v) polyethylene glycol 4000, 0.2 M lithium sulfate, 0.1 M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
