3JSV
Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin
Summary for 3JSV
Entry DOI | 10.2210/pdb3jsv/pdb |
Related | 2ZVN 2ZVO 3F89 3FX0 |
Descriptor | Ubiquitin, NF-kappa-B essential modulator, ... (4 entities in total) |
Functional Keywords | ubiquitin, coiled-coil, cellular signaling, cytoplasm, isopeptide bond, nucleus, phosphoprotein, ubl conjugation, coiled coil, disulfide bond, metal-binding, transcription, transcription regulation, zinc, zinc-finger, signaling protein-transcription complex, signaling protein/transcription |
Biological source | Homo sapiens (human) More |
Cellular location | Cytoplasm (By similarity): O88522 |
Total number of polymer chains | 4 |
Total formula weight | 39508.04 |
Authors | Yoshikawa, A.,Sato, Y.,Mimura, H.,Yamashita, M.,Yamagata, A.,Fukai, S. (deposition date: 2009-09-11, release date: 2009-10-27, Last modification date: 2024-10-30) |
Primary citation | Yoshikawa, A.,Sato, Y.,Yamashita, M.,Mimura, H.,Yamagata, A.,Fukai, S. Crystal structure of the NEMO ubiquitin-binding domain in complex with Lys 63-linked di-ubiquitin Febs Lett., 583:3317-3322, 2009 Cited by PubMed Abstract: NEMO is essential for activation of the NF-kappaB signaling pathway, which is regulated by ubiquitination of proteins. The C-terminal leucine zipper of NEMO and its adjacent coiled-coil region (CC2-LZ) reportedly bind to linear ubiquitin chains with 1 microM affinity and to Lys 63-linked chains with 100 microM affinity. Here we report the crystal structure of the CC2-LZ region of mouse NEMO in complex with Lys 63-linked di-ubiquitin (K63-Ub(2)) at 2.7A resolution. The ubiquitin-binding region consists of a 130A-long helix and forms a parallel coiled-coil dimer. The Ile 44-centered hydrophobic patch of ubiquitin is recognized in the middle of the NEMO ubiquitin-binding region. NEMO interacts with each K63-Ub(2)via a single ubiquitin-binding site, consistent with low affinity binding with K63-Ub(2). PubMed: 19766637DOI: 10.1016/j.febslet.2009.09.028 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
Download full validation report