3JSV
Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.380, 45.870, 83.290 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 49.500 - 2.700 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 11492 | |
| <I/σ(I)> | 14.2 | 3.8 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
