3JSV
Crystal structure of mouse NEMO CoZi in complex with Lys63-linked di-ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.380, 45.870, 83.290 |
Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
Resolution | 49.500 - 2.700 |
R-factor | 0.25 |
Rwork | 0.250 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 11492 | |
<I/σ(I)> | 14.2 | 3.8 |
Completeness [%] | 98.8 | 97.9 |
Redundancy | 31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |