3J6B

Structure of the yeast mitochondrial large ribosomal subunit

This is a non-PDB format compatible entry.

Summary for 3J6B

• Entry DOI: 10.2210/pdb3j6b/pdb
• EMDB information: 2566
• Descriptor: 21S ribosomal RNA, 54S ribosomal protein L10, mitochondrial, 54S ribosomal protein L16, mitochondrial, ... (44 entities in total)
• Functional Keywords: mitochondrial ribosome, large subunit, protein-rna complex, ribosome
• Biological source: Saccharomyces cerevisiae (yeast); More
• Total number of polymer chains: 41
• Total formula weight: 2040157.94

Authors

Amunts, A.,Brown, A.,Bai, X.C.,Llacer, J.L.,Hussain, T.,Emsley, P.,Long, F.,Murshudov, G.,Scheres, S.H.W.,Ramakrishnan, V. (deposition date: 2014-01-22, release date: 2014-04-09, Last modification date: 2024-02-21)

Primary citation

Amunts, A.,Brown, A.,Bai, X.C.,Llacer, J.L.,Hussain, T.,Emsley, P.,Long, F.,Murshudov, G.,Scheres, S.H.,Ramakrishnan, V.
Structure of the yeast mitochondrial large ribosomal subunit.
Science, 343:1485-1489, 2014

PubMed Abstract: Mitochondria have specialized ribosomes that have diverged from their bacterial and cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large subunit using single-particle cryo-electron microscopy. The resolution of 3.2 angstroms enabled a nearly complete atomic model to be built de novo and refined, including 39 proteins, 13 of which are unique to mitochondria, as well as expansion segments of mitoribosomal RNA. The structure reveals a new exit tunnel path and architecture, unique elements of the E site, and a putative membrane docking site.

PubMed: 24675956
DOI: 10.1126/science.1249410

Experimental method

ELECTRON MICROSCOPY (3.2 Å)