3I5D
Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 3.5 Angstroms (R3)
Summary for 3I5D
| Entry DOI | 10.2210/pdb3i5d/pdb |
| Descriptor | P2X purinoceptor, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | p2x, purinergic receptor, ion channel, closed state, apo state, ion transport, ionic channel, receptor, transmembrane, transport, transport protein |
| Biological source | Danio rerio (leopard danio,zebra danio,zebra fish) |
| Total number of polymer chains | 3 |
| Total formula weight | 123645.30 |
| Authors | Kawate, T.,Michel, J.C.,Gouaux, E. (deposition date: 2009-07-05, release date: 2009-08-04, Last modification date: 2024-11-27) |
| Primary citation | Kawate, T.,Michel, J.C.,Birdsong, W.T.,Gouaux, E. Crystal structure of the ATP-gated P2X(4) ion channel in the closed state. Nature, 460:592-598, 2009 Cited by PubMed Abstract: P2X receptors are cation-selective ion channels gated by extracellular ATP, and are implicated in diverse physiological processes, from synaptic transmission to inflammation to the sensing of taste and pain. Because P2X receptors are not related to other ion channel proteins of known structure, there is at present no molecular foundation for mechanisms of ligand-gating, allosteric modulation and ion permeation. Here we present crystal structures of the zebrafish P2X(4) receptor in its closed, resting state. The chalice-shaped, trimeric receptor is knit together by subunit-subunit contacts implicated in ion channel gating and receptor assembly. Extracellular domains, rich in beta-strands, have large acidic patches that may attract cations, through fenestrations, to vestibules near the ion channel. In the transmembrane pore, the 'gate' is defined by an approximately 8 A slab of protein. We define the location of three non-canonical, intersubunit ATP-binding sites, and suggest that ATP binding promotes subunit rearrangement and ion channel opening. PubMed: 19641588DOI: 10.1038/nature08198 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.46 Å) |
Structure validation
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