3I5D
Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 3.5 Angstroms (R3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-01-01 |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 |
Unit cell lengths | 234.910, 234.910, 138.433 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.740 - 3.460 |
R-factor | 0.277 |
Rwork | 0.276 |
R-free | 0.28900 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.272 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.520 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.053 | 0.791 |
Number of reflections | 43062 | |
<I/σ(I)> | 18.1 | 1.4 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.4 | 277 | 12 % PEG 4,000, 100 mM sodium acetate, 100 mM ammonium sulfate, 1 mM GdCl3, pH 4.4, vapor diffusion, temperature 277K |