3EQL

Crystal structure of the T. Thermophilus RNA polymerase holoenzyme in complex with antibiotic myxopyronin

Summary for 3EQL

Related1IW7 1SMY 2A6E 2A69 2A68 2A6H 2BE5
DescriptorDNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (9 entities in total)
Functional Keywordsrna polymerase holoenzyme, myxopyronin, antibiotic, transcription regulation, dna-directed rna polymerase, nucleotidyltransferase, transcription, transferase, dna-binding, sigma factor
Biological sourceThermus thermophilus
Total number of polymer chains12
Total molecular weight854440.4
Authors
Vassylyev, D.G.,Vassylyeva, M.N.,Artsimovitch, I. (deposition date: 2008-09-30, release date: 2008-10-28, Last modification date: 2011-07-13)
Primary citation
Belogurov, G.A.,Vassylyeva, M.N.,Sevostyanova, A.,Appleman, J.R.,Xiang, A.X.,Lira, R.,Webber, S.E.,Klyuyev, S.,Nudler, E.,Artsimovitch, I.,Vassylyev, D.G.
Transcription inactivation through local refolding of the RNA polymerase structure.
Nature, 457:332-335, 2009
PubMed: 18946472 (PDB entries with the same primary citation)
DOI: 10.1038/nature07510
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.7 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.260574.2%17.6%1.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3eql
no rotation
Molmil generated image of 3eql
rotated about x axis by 90°
Molmil generated image of 3eql
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 3eql
no rotation
Molmil generated image of 3eql
rotated about x axis by 90°
Molmil generated image of 3eql
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3eql.pdb2.gz [586.23 KB])
Coordinate files for Biological unit (3eql.pdb1.gz [587.62 KB])