3KHU

Crystal structure of human UDP-glucose dehydrogenase Glu161Gln, in complex with thiohemiacetal intermediate

Summary for 3KHU

• Entry DOI: 10.2210/pdb3khu/pdb
• Related: 2Q3E 2QG4 3ITK
• Descriptor: UDP-glucose 6-dehydrogenase, URIDINE-5'-DIPHOSPHATE-GLUCOSE, 1,2-ETHANEDIOL, ... (5 entities in total)
• Functional Keywords: oxidoreductase, thiohemiacetal intermediate, structural genomics, structural genomics consortium, sgc
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 6
• Total formula weight: 320583.69

Authors

Chaikuad, A.,Egger, S.,Yue, W.W.,Guo, K.,Sethi, R.,Filippakopoulos, P.,Muniz, J.R.C.,von Delft, F.,Bountra, C.,Arrowsmith, C.H.,Weigelt, J.,Edwards, A.M.,Kavanagh, K.L.,Nidetzky, B.,Oppermann, U.,Structural Genomics Consortium (SGC) (deposition date: 2009-10-30, release date: 2009-11-17, Last modification date: 2023-09-06)

• Primary citation: Egger, S.,Chaikuad, A.,Klimacek, M.,Kavanagh, K.L.,Oppermann, U.,Nidetzky, B.; Structural and Kinetic Evidence That Catalytic Reaction of Human UDP-glucose 6-Dehydrogenase Involves Covalent Thiohemiacetal and Thioester Enzyme Intermediates.; J.Biol.Chem., 287:2119-2129, 2012; PubMed: 22123821; DOI: 10.1074/jbc.M111.313015

Experimental method

X-RAY DIFFRACTION (2.3 Å)