2YCX

TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND ANTAGONIST CYANOPINDOLOL

2YCX の概要

• エントリーDOI: 10.2210/pdb2ycx/pdb
• 関連するPDBエントリー: 1DEP 2VT4 2Y00 2Y01 2Y02 2Y03 2Y04 2YCW 2YCY 2YCZ
• 分子名称: BETA-1 ADRENERGIC RECEPTOR, Cyanopindolol, octyl 1-thio-beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: receptor, transducer, antagonist bound form, integral membrane protein, g-protein coupled receptor, thermostabilising point mutations, seven-helix receptor, 7tm receptor, gpcr
• 由来する生物種: MELEAGRIS GALLOPAVO (TURKEY)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 73005.21

構造登録者

Moukhametzianov, R.,Warne, T.,Edwards, P.C.,Serrano-Vega, M.J.,Leslie, A.G.W.,Tate, C.G.,Schertler, G.F.X. (登録日: 2011-03-17, 公開日: 2011-06-01, 最終更新日: 2025-07-16)

主引用文献

Moukhametzianov, R.,Warne, T.,Edwards, P.C.,Serrano-Vega, M.J.,Leslie, A.G.,Tate, C.G.,Schertler, G.F.
Two Distinct Conformations of Helix 6 Observed in Antagonist-Bound Structures of a Beta-1- Adrenergic Receptor.
Proc.Natl.Acad.Sci.USA, 108:8228-, 2011

PubMed Abstract: The β(1)-adrenergic receptor (β(1)AR) is a G-protein-coupled receptor whose inactive state structure was determined using a thermostabilized mutant (β(1)AR-M23). However, it was not thought to be in a fully inactivated state because there was no salt bridge between Arg139 and Glu285 linking the cytoplasmic ends of transmembrane helices 3 and 6 (the R(3.50) - D/E(6.30) "ionic lock"). Here we compare eight new structures of β(1)AR-M23, determined from crystallographically independent molecules in four different crystals with three different antagonists bound. These structures are all in the inactive R state and show clear electron density for cytoplasmic loop 3 linking transmembrane helices 5 and 6 that had not been seen previously. Despite significantly different crystal packing interactions, there are only two distinct conformations of the cytoplasmic end of helix 6, bent and straight. In the bent conformation, the Arg139-Glu285 salt bridge is present, as in the crystal structure of dark-state rhodopsin. The straight conformation, observed in previously solved structures of β-receptors, results in the ends of helices 3 and 6 being too far apart for the ionic lock to form. In the bent conformation, the R(3.50)-E(6.30) distance is significantly longer than in rhodopsin, suggesting that the interaction is also weaker, which could explain the high basal activity in β(1)AR compared to rhodopsin. Many mutations that increase the constitutive activity of G-protein-coupled receptors are found in the bent region at the cytoplasmic end of helix 6, supporting the idea that this region plays an important role in receptor activation.

PubMed: 21540331
DOI: 10.1073/PNAS.1100185108

実験手法

X-RAY DIFFRACTION (3.25 Å)