2MEJ

Solution Structure of the Complex Between BCL-xL and the p53 Core Domain determined with PRE restraints

Summary for 2MEJ

• Entry DOI: 10.2210/pdb2mej/pdb
• Related: 2AC0 2ME8 2ME9
• NMR Information: BMRB: 19522
• Descriptor: Bcl-2-like protein 1, Cellular tumor antigen p53, ZINC ION (3 entities in total)
• Functional Keywords: bcl-xl, p53, apoptosis, bcl-2 family, cytoplasmic p53, selective labeling
• Biological source: Homo sapiens (human); More
• Cellular location: Isoform Bcl-X(L): Mitochondrion inner membrane : Q07817; Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• Total number of polymer chains: 2
• Total formula weight: 48148.07

Authors

Viacava Follis, A.,Grace, C.R.,Kriwacki, R.W. (deposition date: 2013-09-25, release date: 2014-04-30, Last modification date: 2024-05-15)

Primary citation

Follis, A.V.,Llambi, F.,Ou, L.,Baran, K.,Green, D.R.,Kriwacki, R.W.
The DNA-binding domain mediates both nuclear and cytosolic functions of p53.
Nat.Struct.Mol.Biol., 21:535-543, 2014

PubMed Abstract: Under conditions of genotoxic stress, human p53 activates the apoptotic effectors BAX or BAK to result in mitochondrial outer-membrane permeabilization and apoptosis. Antiapoptotic BCL-2 family member BCL-xL opposes this activity by sequestering cytosolic p53 via association with its DNA-binding domain, an interaction enhanced by p53 tetramerization. Here we characterized the BCL-xL-p53 complex by NMR spectroscopy and modulated it through mutagenesis to determine the relative contributions of BCL-xL's interactions with p53 or other BCL-2 family proteins to the BCL-xL-dependent inhibition of UV irradiation-induced apoptosis. Under our experimental conditions, one-third of the antiapoptotic activity of BCL-xL was mediated by p53 sequestration and the remaining two-thirds through sequestration of proapoptotic BCL-2 family members. Our studies define the contributions of cytosolic p53 to UV irradiation-induced apoptosis and provide opportunities to explore its contributions to other p53-dependent apoptotic signaling pathways.

PubMed: 24814347
DOI: 10.1038/nsmb.2829

Experimental method

SOLUTION NMR