2AKR

Structural basis of sulfatide presentation by mouse CD1d

Summary for 2AKR

• Entry DOI: 10.2210/pdb2akr/pdb
• Related: 1CD1 1Z5L 1ZHN 1ZT4
• Descriptor: T-cell surface glycoprotein CD1d1, Beta-2-microglobulin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: nkt cells, cd1d, mhc fold, sulfatide, self-antigen, tcr, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 91897.08

Authors

Zajonc, D.M.,Halder, R.,Wu, D.,Maricic, I.,Roy, K.,Wong, C.-H.,Kumar, V.,Wilson, I.A. (deposition date: 2005-08-03, release date: 2005-12-06, Last modification date: 2024-11-20)

Primary citation

Zajonc, D.M.,Maricic, I.,Wu, D.,Halder, R.,Roy, K.,Wong, C.-H.,Kumar, V.,Wilson, I.A.
Structural basis for CD1d presentation of a sulfatide derived from myelin and its implications for autoimmunity
J.Exp.Med., 202:1517-1526, 2005

PubMed Abstract: Sulfatide derived from the myelin stimulates a distinct population of CD1d-restricted natural killer T (NKT) cells. Cis-tetracosenoyl sulfatide is one of the immunodominant species in myelin as identified by proliferation, cytokine secretion, and CD1d tetramer staining. The crystal structure of mouse CD1d in complex with cis-tetracosenoyl sulfatide at 1.9 A resolution reveals that the longer cis-tetracosenoyl fatty acid chain fully occupies the A' pocket of the CD1d binding groove, whereas the sphingosine chain fills up the F' pocket. A precise hydrogen bond network in the center of the binding groove orients and positions the ceramide backbone for insertion of the lipid tails in their respective pockets. The 3'-sulfated galactose headgroup is highly exposed for presentation to the T cell receptor and projects up and away from the binding pocket due to its beta linkage, compared with the more intimate binding of the alpha-glactosyl ceramide headgroup to CD1d. These structure and binding data on sulfatide presentation by CD1d have important implications for the design of therapeutics that target T cells reactive for myelin glycolipids in autoimmune diseases of the central nervous system.

PubMed: 16314439
DOI: 10.1084/jem.20051625

Experimental method

X-RAY DIFFRACTION (1.9 Å)