2AKR
Structural basis of sulfatide presentation by mouse CD1d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-04-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.858, 74.843, 101.564 |
Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
Resolution | 41.380 - 1.900 |
R-factor | 0.187 |
Rwork | 0.186 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z5l |
RMSD bond length | 0.016 |
RMSD bond angle | 1.749 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 67171 | |
<I/σ(I)> | 15.9 | 2.4 |
Completeness [%] | 98.7 | 99.7 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 277 | ammonium sulfate, NaCl, sodium cacodylate, manganese chloride, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K, pH 6.50 |