2YE0
X-ray structure of the cyan fluorescent protein mTurquoise (K206A mutant)
Summary for 2YE0
| Entry DOI | 10.2210/pdb2ye0/pdb |
| Related | 1B9C 1BFP 1C4F 1CV7 1EMA 1EMB 1EMC 1EME 1EMF 1EMG 1EMK 1EML 1EMM 1F09 1F0B 1GFL 1H6R 1HCJ 1HUY 1JBY 1JBZ 1JC0 1JC1 1KP5 1KYP 1KYR 1KYS 1MYW 1Q4A 1Q4B 1Q4C 1Q4D 1Q4E 1Q73 1QXT 1QY3 1QYF 1QYO 1QYQ 1RM9 1RMM 1RMO 1RMP 1RRX 1W7S 1W7T 1W7U 1YFP 1YHG 1YHH 1YHI 1YJ2 1YJF 1Z1P 1Z1Q 2AH8 2AHA 2B3P 2B3Q 2EMD 2EMN 2EMO 2FWQ 2FZU 2WSN 2WSO 2WUR 2Y0G 2YDZ 2YE1 2YFP 3ZTF |
| Descriptor | GREEN FLUORESCENT PROTEIN (2 entities in total) |
| Functional Keywords | fluorescent protein, fret donor |
| Biological source | AEQUOREA VICTORIA |
| Total number of polymer chains | 1 |
| Total formula weight | 27666.16 |
| Authors | von Stetten, D.,Goedhart, J.,Noirclerc-Savoye, M.,Lelimousin, M.,Joosen, L.,Hink, M.A.,van Weeren, L.,Gadella, T.W.J.,Royant, A. (deposition date: 2011-03-25, release date: 2012-03-21, Last modification date: 2024-11-20) |
| Primary citation | Goedhart, J.,von Stetten, D.,Noirclerc-Savoye, M.,Lelimousin, M.,Joosen, L.,Hink, M.A.,van Weeren, L.,Gadella, T.W.J.,Royant, A. Structure-Guided Evolution of Cyan Fluorescent Proteins Towards a Quantum Yield of 93% Nat.Commun, 3:751-, 2012 Cited by PubMed Abstract: Cyan variants of green fluorescent protein are widely used as donors in Förster resonance energy transfer experiments. The popular, but modestly bright, Enhanced Cyan Fluorescent Protein (ECFP) was sequentially improved into the brighter variants Super Cyan Fluorescent Protein 3A (SCFP3A) and mTurquoise, the latter exhibiting a high-fluorescence quantum yield and a long mono-exponential fluorescence lifetime. Here we combine X-ray crystallography and excited-state calculations to rationalize these stepwise improvements. The enhancement originates from stabilization of the seventh β-strand and the strengthening of the sole chromophore-stabilizing hydrogen bond. The structural analysis highlighted one suboptimal internal residue, which was subjected to saturation mutagenesis combined with fluorescence lifetime-based screening. This resulted in mTurquoise2, a brighter variant with faster maturation, high photostability, longer mono-exponential lifetime and the highest quantum yield measured for a monomeric fluorescent protein. Together, these properties make mTurquoise2 the preferable cyan variant of green fluorescent protein for long-term imaging and as donor for Förster resonance energy transfer to a yellow fluorescent protein. PubMed: 22434194DOI: 10.1038/NCOMMS1738 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.47 Å) |
Structure validation
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