1Z25

Structure of P.furiosus Argonaute with bound Mn2+

1Z25 の概要

• エントリーDOI: 10.2210/pdb1z25/pdb
• 関連するPDBエントリー: 1U04 1Z26
• 分子名称: Argonaute, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: argonaute, piwi, active site, rnaseh, mg2+, rnai, gene regulation
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 90652.11

構造登録者

Rivas, F.V.,Tolia, N.H.,Song, J.J.,Aragon, J.P.,Liu, J.,Hannon, G.J.,Joshua-Tor, L. (登録日: 2005-03-07, 公開日: 2005-04-05, 最終更新日: 2024-02-14)

主引用文献

Rivas, F.V.,Tolia, N.H.,Song, J.J.,Aragon, J.P.,Liu, J.,Hannon, G.J.,Joshua-Tor, L.
Purified Argonaute2 and an siRNA form recombinant human RISC.
Nat.Struct.Mol.Biol., 12:340-349, 2005

PubMed Abstract: Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector complex, RISC. Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that accurately cleaves substrate RNAs. Recombinant RISC shows many of the properties of RISC purified from human or Drosophila melanogaster cells but also has surprising features. It shows no stimulation by ATP, suggesting that factors promoting product release are missing from the recombinant enzyme. The active site is made up of a unique Asp-Asp-His (DDH) motif. In the RISC reconstitution system, the siRNA 5' phosphate is important for the stability and the fidelity of the complex but is not essential for the creation of an active enzyme. These studies demonstrate that Argonaute proteins catalyze mRNA cleavage within RISC and provide a source of recombinant enzyme for detailed biochemical studies of the RNAi effector complex.

PubMed: 15800637
DOI: 10.1038/nsmb918

実験手法

X-RAY DIFFRACTION (2.7 Å)