1Z25
Structure of P.furiosus Argonaute with bound Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.662, 105.108, 73.662 |
| Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
| Resolution | 42.420 - 2.700 |
| R-factor | 0.26 |
| Rwork | 0.215 |
| R-free | 0.27400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 27689 | |
| <I/σ(I)> | 26.4 | 3.7 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | alcohol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
