1UVP

Structure Of The Complex Of Porcine Pancreatic Elastase In Complex With Cadmium Refined At 1.85 A Resolution (Crystal B)

Summary for 1UVP

• Entry DOI: 10.2210/pdb1uvp/pdb
• Related: 1B0E 1BMA 1BTU 1C1M 1E34 1E35 1E36 1E37 1E38 1EAI 1EAS 1EAT 1EAU 1ELA 1ELB 1ELC 1ELD 1ELE 1ELF 1ELG 1ESA 1ESB 1EST 1FLE 1FZZ 1GVK 1GWA 1H9L 1HAX 1HAY 1HAZ 1HB0 1HV7 1INC 1JIM 1L0Z 1L1G 1LKA 1LKB 1LVY 1MCV 1MMJ 1NES 1OKX 1QGF 1QIX 1QNJ 1QR3 1UO6 1UVO 2EST 3EST 4EST 5EST 6EST 7EST 8EST 9EST
• Descriptor: ELASTASE 1, CADMIUM ION, ACETATE ION, ... (6 entities in total)
• Functional Keywords: hydrolase, chymotrypsin-fold, beta-barrel, serine protease
• Biological source: SUS SCROFA (PIG)
• Cellular location: Secreted: P00772
• Total number of polymer chains: 1
• Total formula weight: 26228.02

Authors

Weiss, M.S.,Panjikar, S.,Mueller-Dieckmann, C.,Tucker, P.A. (deposition date: 2004-01-21, release date: 2004-02-10, Last modification date: 2024-11-13)

Primary citation

Weiss, M.S.,Panjikar, S.,Mueller-Dieckmann, C.,Tucker, P.A.
On the Influence of the Incident Photon Energy on the Radiation Damage in Crystalline Biological Samples
J.Synchrotron Radiat., 12:304-, 2005

PubMed Abstract: Two series of complete and highly redundant data sets were collected at wavelengths of 1.00 and 2.00 Angstroms on a cadmium derivative of porcine pancreatic elastase (PPE). Radiation damage to the sample was evaluated qualitatively by inspecting consecutive difference electron density maps during the course of the experiment. The nature of the radiation damage was found to be identical at both wavelengths and was localized primarily at the four disulfide bridges of PPE, the cadmium site and the two methionine residues. For a quantitative examination of the radiation damage, the decrease in the peak height of the cadmium ion in various electron density maps was exploited. Again, no significant difference in radiation damage between the two wavelengths was observed. This can be rationalized by considering the wavelength dependencies of the number of diffracted photons versus the number of absorbed photons and the energy deposited in the crystal by the latter.

PubMed: 15840915
DOI: 10.1107/S0909049505003328

Experimental method

X-RAY DIFFRACTION (1.85 Å)