1UO6

PORCINE PANCREATIC ELASTASE/Xe-COMPLEX

Summary for 1UO6

• Entry DOI: 10.2210/pdb1uo6/pdb
• Related: 1B0E 1BMA 1BTU 1C1M 1E34 1E35 1E36 1E37 1E38 1EAI 1EAS 1EAT 1EAU 1ELA 1ELB 1ELC 1ELD 1ELE 1ELF 1ELG 1ESA 1ESB 1EST 1FLE 1FZZ 1GVK 1GWA 1H9L 1HAX 1HAY 1HAZ 1HB0 1HV7 1INC 1JIM 1L0Z 1L1G 1LKA 1LKB 1LVY 1MCV 1MMJ 1NES 1OKX 1QGF 1QIX 1QNJ 1QR3 2EST 3EST 4EST 5EST 6EST 7EST 8EST 9EST
• Descriptor: ELASTASE 1, GLYCEROL, SULFATE ION, ... (7 entities in total)
• Functional Keywords: hydrolase, chymotrypsin-fold, beta-barrel, xenon binding
• Biological source: SUS SCROFA (PIG)
• Cellular location: Secreted: P00772
• Total number of polymer chains: 1
• Total formula weight: 26402.97

Authors

Mueller-Dieckmann, C.,Polentarutti, M.,Djinovic-Carugo, K.,Panjikar, S.,Tucker, P.A.,Weiss, M.S. (deposition date: 2003-09-16, release date: 2003-10-14, Last modification date: 2023-12-13)

Primary citation

Mueller-Dieckmann, C.,Polentarutti, M.,Djinovic-Carugo, K.,Panjikar, S.,Tucker, P.A.,Weiss, M.S.
On the Routine Use of Soft X-Rays in Macromolecular Crystallography. Part II. Data-Collection Wavelength and Scaling Models
Acta Crystallogr.,Sect.D, 60:28-, 2004

PubMed Abstract: Complete and highly redundant data sets were collected at nine different wavelengths between 0.80 and 2.65 A on a xenon derivative of porcine pancreatic elastase in both air and helium atmospheres. The magnitude of the anomalous signal, as assessed by the xenon-peak height in the anomalous difference Patterson synthesis, is affected by the wavelength of data collection as well as by the scaling model used. For data collected at wavelengths longer than 1.7 A, the use of a three-dimensional scaling protocol is essential in order to obtain the highest possible anomalous signal. Based on the scaling protocols currently available, the optimal wavelength range for data collection appears to be between 2.1 and 2.4 A. Beyond that, any further increase in signal will be compensated for or even superseded by a concomitant increase in noise, which cannot be fully corrected for. Data collection in a helium atmosphere yields higher I/sigma(I) values, but not significantly better anomalous differences, than data collection in air.

PubMed: 14684889
DOI: 10.1107/S0907444903020833

Experimental method

X-RAY DIFFRACTION (1.65 Å)