1TN8

Protein Farnesyltransferase Complexed with a H-Ras Peptide Substrate and a FPP Analog at 2.25A Resolution

Summary for 1TN8

• Entry DOI: 10.2210/pdb1tn8/pdb
• Related: 1D8D 1FPP 1FT1 1N4Q 1QBQ 1TN6 1TN7 1TNB 1TNO 1TNU 1TNY 1TNZ
• Descriptor: Protein farnesyltransferase/geranylgeranyltransferase type I alpha subunit, Protein farnesyltransferase beta subunit, peptide derived from the C-terminus of H-Ras, ... (7 entities in total)
• Functional Keywords: ftase, farnesyltransferase, farnesyl transferase, prenyltransferase, caax, ras, lipid modification, prenylation, substrate selectivity, transferase
• Biological source: Rattus norvegicus (Norway rat); More
• Total number of polymer chains: 3
• Total formula weight: 93782.86

Authors

Reid, T.S.,Terry, K.L.,Casey, P.J.,Beese, L.S. (deposition date: 2004-06-11, release date: 2004-11-02, Last modification date: 2023-08-23)

Primary citation

Reid, T.S.,Terry, K.L.,Casey, P.J.,Beese, L.S.
Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity.
J.Mol.Biol., 343:417-433, 2004

PubMed Abstract: Post-translational modifications are essential for the proper function of many proteins in the cell. The attachment of an isoprenoid lipid (a process termed prenylation) by protein farnesyltransferase (FTase) or geranylgeranyltransferase type I (GGTase-I) is essential for the function of many signal transduction proteins involved in growth, differentiation, and oncogenesis. FTase and GGTase-I (also called the CaaX prenyltransferases) recognize protein substrates with a C-terminal tetrapeptide recognition motif called the Ca1a2X box. These enzymes possess distinct but overlapping protein substrate specificity that is determined primarily by the sequence identity of the Ca1a2X motif. To determine how the identity of the Ca1a2X motif residues and sequence upstream of this motif affect substrate binding, we have solved crystal structures of FTase and GGTase-I complexed with a total of eight cognate and cross-reactive substrate peptides, including those derived from the C termini of the oncoproteins K-Ras4B, H-Ras and TC21. These structures suggest that all peptide substrates adopt a common binding mode in the FTase and GGTase-I active site. Unexpectedly, while the X residue of the Ca1a2X motif binds in the same location for all GGTase-I substrates, the X residue of FTase substrates can bind in one of two different sites. Together, these structures outline a series of rules that govern substrate peptide selectivity; these rules were utilized to classify known protein substrates of CaaX prenyltransferases and to generate a list of hypothetical substrates within the human genome.

PubMed: 15451670
DOI: 10.1016/j.jmb.2004.08.056

Experimental method

X-RAY DIFFRACTION (2.25 Å)