1SL0

Ternary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template and an incoming nucleotide

1SL0 の概要

• エントリーDOI: 10.2210/pdb1sl0/pdb
• 関連するPDBエントリー: 1SKR 1SKS 1SKW 1SL1 1SL2 1T7P
• 分子名称: 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*(2DT))-3', 5'-D(*CP*CP*CP*(TTD)P*AP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3', DNA polymerase, ... (6 entities in total)
• 機能のキーワード: dna polymerase, fidelity, lesion bypass, thymine dimer, open, close, transferase-electron transport-dna complex, transferase/electron transport/dna
• 由来する生物種: Enterobacteria phage T7; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 211249.84

構造登録者

Li, Y.,Dutta, S.,Doublie, S.,Bdour, H.M.,Taylor, J.S.,Ellenberger, T. (登録日: 2004-03-05, 公開日: 2004-07-06, 最終更新日: 2024-02-14)

主引用文献

Li, Y.,Dutta, S.,Doublie, S.,Bdour, H.M.,Taylor, J.S.,Ellenberger, T.
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.
Nat.Struct.Mol.Biol., 11:784-790, 2004

PubMed Abstract: Ultraviolet-induced DNA damage poses a lethal block to replication. To understand the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (CPD). When the 3' thymine is the templating base, the CPD is rotated out of the polymerase active site and the fingers subdomain adopts an open orientation. When the 5' thymine is the templating base, the CPD lies within the polymerase active site where it base-pairs with the incoming nucleotide and the 3' base of the primer, while the fingers are in a closed conformation. These structures reveal the basis for the strong block of DNA replication that is caused by this photolesion.

PubMed: 15235589
DOI: 10.1038/nsmb792

実験手法

X-RAY DIFFRACTION (3.2 Å)