1SL0

Ternary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template and an incoming nucleotide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (P, Q)	5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*(2DT))-3'	polymer	21	6426.2	2
2	B, D (T, U)	5'-D(*CP*CP*CP*(TTD)P*AP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3'	polymer	25	7922.1	2
3	E, G (A, C)	DNA polymerase	polymer	698	79089.8	2	UniProt (P00581) Pfam (PF00476)	Enterobacteria phage T7	T7 DNA polymerase
4	F, H (B, D)	Thioredoxin 1	polymer	108	11687.4	2	UniProt (P0AA25) Pfam (PF00085)	Escherichia coli	TRX1, TRX
5	I, K (A, C)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	J, L (A, C)	2',3'-DIDEOXYADENOSINE-5'-TRIPHOSPHATE	non-polymer		475.2	2	Chemie (DAD)

Sequence modifications

A, C: 1 - 704 (UniProt: P00581)

PDB	External Database	Details
-	Lys 118	deletion
-	Arg 119	deletion
-	Phe 120	deletion
-	Gly 121	deletion
-	Ser 122	deletion
-	His 123	deletion

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	210250.9
Non-Polymers*	Number of molecules	4
	Total formula weight	999.0
All*	Total formula weight	211249.8

*Water molecules are not included.