1SL0
Ternary 3' complex of T7 DNA polymerase with a DNA primer/template containing a disordered cis-syn thymine dimer on the template and an incoming nucleotide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| A | 0004527 | molecular_function | exonuclease activity |
| A | 0004529 | molecular_function | DNA exonuclease activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0006260 | biological_process | DNA replication |
| A | 0006261 | biological_process | DNA-templated DNA replication |
| A | 0006302 | biological_process | double-strand break repair |
| A | 0008408 | molecular_function | 3'-5' exonuclease activity |
| A | 0039693 | biological_process | viral DNA genome replication |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0090592 | biological_process | DNA synthesis involved in DNA replication |
| B | 0005515 | molecular_function | protein binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0015035 | molecular_function | protein-disulfide reductase activity |
| B | 0015036 | molecular_function | disulfide oxidoreductase activity |
| B | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| B | 0045454 | biological_process | cell redox homeostasis |
| C | 0000166 | molecular_function | nucleotide binding |
| C | 0003676 | molecular_function | nucleic acid binding |
| C | 0003677 | molecular_function | DNA binding |
| C | 0003887 | molecular_function | DNA-directed DNA polymerase activity |
| C | 0004527 | molecular_function | exonuclease activity |
| C | 0004529 | molecular_function | DNA exonuclease activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0006260 | biological_process | DNA replication |
| C | 0006261 | biological_process | DNA-templated DNA replication |
| C | 0006302 | biological_process | double-strand break repair |
| C | 0008408 | molecular_function | 3'-5' exonuclease activity |
| C | 0039693 | biological_process | viral DNA genome replication |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0090592 | biological_process | DNA synthesis involved in DNA replication |
| D | 0005515 | molecular_function | protein binding |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0015035 | molecular_function | protein-disulfide reductase activity |
| D | 0015036 | molecular_function | disulfide oxidoreductase activity |
| D | 0030337 | molecular_function | DNA polymerase processivity factor activity |
| D | 0045454 | biological_process | cell redox homeostasis |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE MG A 4003 |
| Chain | Residue |
| A | ASP5 |
| A | ASP65 |
| site_id | AC2 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG C 4004 |
| Chain | Residue |
| C | ASP5 |
| C | HIS59 |
| C | ASP65 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE DAD C 4005 |
| Chain | Residue |
| C | HIS506 |
| C | ARG518 |
| site_id | AC4 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE DAD A 4004 |
| Chain | Residue |
| A | TYR526 |
| A | HIS506 |
| A | ARG518 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Nucleophile |
| Chain | Residue | Details |
| B | GLY33 | |
| B | LYS36 | |
| D | GLY33 | |
| D | LYS36 | |
| C | GLU7 | |
| C | ALA180 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | SITE: Deprotonates C-terminal active site Cys |
| Chain | Residue | Details |
| B | PHE27 | |
| D | PHE27 | |
| A | CYS660 | |
| C | LEU481 | |
| C | ARG482 | |
| C | CYS660 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Contributes to redox potential value |
| Chain | Residue | Details |
| B | PRO34 | |
| B | CYS35 | |
| D | PRO34 | |
| D | CYS35 | |
| C | ALA512 | |
| C | PHE524 | |
| C | PHE528 | |
| C | ALA532 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:18723842 |
| Chain | Residue | Details |
| B | TYR70 | |
| D | TYR70 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| B | PRO34 | |
| B | CYS35 | |
| B | CYS32 | |
| B | GLY33 |
| site_id | CSA2 |
| Number of Residues | 4 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| D | PRO34 | |
| D | CYS35 | |
| D | CYS32 | |
| D | GLY33 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| B | CYS35 | |
| B | CYS32 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1mek |
| Chain | Residue | Details |
| D | CYS35 | |
| D | CYS32 |
