1SKS

Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template

Summary for 1SKS

Related1T7P 1SKR 1SKW 1SL0 1SL1 1SL2
Descriptor5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*AP*C*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*(2DT))-3', 5'-D(*CP*CP*CP*(TTD)P*AP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3', DNA polymerase, ... (6 entities in total)
Functional Keywordsdna polymerase, lesion bypass, fidelity, open, close, transferase-electron transport-dna complex, transferase/electron transport/dna
Biological sourceEnterobacteria phage T7
More
Total number of polymer chains4
Total molecular weight105149.74
Authors
Li, Y.,Dutta, S.,Doublie, S.,Bdour, H.M.,Taylor, J.S.,Ellenberger, T. (deposition date: 2004-03-05, release date: 2004-07-06, Last modification date: 2011-07-13)
Primary citation
Li, Y.,Dutta, S.,Doublie, S.,Bdour, H.M.,Taylor, J.S.,Ellenberger, T.
Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7.
Nat.Struct.Mol.Biol., 11:784-790, 2004
PubMed: 15235589 (PDB entries with the same primary citation)
DOI: 10.1038/nsmb792
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
NMR Information
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.272131.3%4.1%4.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report