1SKS
Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-19 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.773, 214.917, 52.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.272 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 53547 | |
<I/σ(I)> | 23.7 | 3.7 |
Completeness [%] | 97.5 | 93.4 |
Redundancy | 24.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | VAPOR DIFFUSION, HANGING DROP, pH 7.5 |