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1SKS

Binary 3' complex of T7 DNA polymerase with a DNA primer/template containing a cis-syn thymine dimer on the template

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCHESS BEAMLINE F1
Synchrotron siteCHESS
BeamlineF1
Temperature [K]100
Detector technologyCCD
Collection date2002-07-19
Spacegroup nameP 21 21 2
Unit cell lengths104.773, 214.917, 52.257
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 2.300
R-factor0.235
Rwork0.235
R-free0.26600
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.007
RMSD bond angle1.272
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareEPMR
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.380
High resolution limit [Å]2.3002.300
Number of reflections53547
<I/σ(I)>23.73.7
Completeness [%]97.593.4
Redundancy24.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
17.5VAPOR DIFFUSION, HANGING DROP, pH 7.5

217705

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