1QMC

C-terminal DNA-binding domain of HIV-1 integrase, NMR, 42 structures

1QMC の概要

• エントリーDOI: 10.2210/pdb1qmc/pdb
• 関連するPDBエントリー: 1A5V 1A5W 1A5X 1AUB 1B9D 1B9F 1BHL 1BI4 1BIS 1BIU 1BL3 1WJA 1WJB 1WJC 1WJD 1WJE 1WJF 2ITG
• 分子名称: HIV-1 INTEGRASE (1 entity in total)
• 機能のキーワード: integrase, dna-binding protein, src homology 3 (sh3)-like fold, aids, polyprotein, transferase
• 由来する生物種: HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 BH10
• 細胞内の位置: Gag-Pol polyprotein: Host cell membrane; Lipid-anchor. Matrix protein p17: Virion membrane; Lipid- anchor . Capsid protein p24: Virion . Nucleocapsid protein p7: Virion . Reverse transcriptase/ribonuclease H: Virion . Integrase: Virion : P03366
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12304.46

構造登録者

Eijkelenboom, A.P.A.M.,Sprangers, R.,Hard, K.,Puras Lutzke, R.A.,Plasterk, R.H.A.,Boelens, R.,Kaptein, R. (登録日: 1999-09-27, 公開日: 1999-12-14, 最終更新日: 2024-05-15)

主引用文献

Eijkelenboom, A.P.A.M.,Sprangers, R.,Hard, K.,Puras Lutzke, R.A.,Plasterk, R.H.A.,Boelens, R.,Kaptein, R.
Refined Solution Structure of the C-Terminal DNA-Binding Domain of Human Immunovirus-1 Integrase.
Proteins: Struct.,Funct., Genet., 36:556-, 1999

PubMed Abstract: The structure of the C-terminal DNA-binding domain of human immunovirus-1 integrase has been refined using nuclear magnetic resonance spectroscopy. The protein is a dimer in solution and shows a well-defined dimer interface. The folding topology of the monomer consists of a five-stranded beta-barrel that resembles that of Src homology 3 domains. Compared with our previously reported structure, the structure is now defined far better. The final 42 structures display a back-bone root mean square deviation versus the average of 0.46 A. Correlation of the structure with recent mutagenesis studies suggests two possible models for DNA binding. Proteins 1999;36:556-564.

PubMed: 10450096
DOI: 10.1002/(SICI)1097-0134(19990901)36:4<556::AID-PROT18>3.3.CO;2-Y

実験手法

SOLUTION NMR