1WJA

SOLUTION STRUCTURE OF THE N-TERMINAL ZN BINDING DOMAIN OF HIV-1 INTEGRASE (D FORM), NMR, REGULARIZED MEAN STRUCTURE

1WJA の概要

• エントリーDOI: 10.2210/pdb1wja/pdb
• 分子名称: HIV-1 INTEGRASE, ZINC ION (2 entities in total)
• 機能のキーワード: zn-binding protein, aids, polyprotein, hydrolase, aspartyl protease, endonuclease
• 由来する生物種: Human immunodeficiency virus 1
• 細胞内の位置: Matrix protein p17: Virion (Potential). Capsid protein p24: Virion (Potential). Nucleocapsid protein p7: Virion (Potential). Reverse transcriptase/ribonuclease H: Virion (Potential). Integrase: Virion (Potential): P35963
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 10786.86

構造登録者

Clore, G.M.,Cai, M.,Caffrey, M.,Gronenborn, A.M. (登録日: 1997-05-13, 公開日: 1998-05-13, 最終更新日: 2024-05-22)

主引用文献

Cai, M.,Zheng, R.,Caffrey, M.,Craigie, R.,Clore, G.M.,Gronenborn, A.M.
Solution structure of the N-terminal zinc binding domain of HIV-1 integrase.
Nat.Struct.Biol., 4:567-577, 1997

PubMed Abstract: The solution structure of the N-terminal zinc binding domain (residues 1-55; IN1-55) of HIV-1 integrase has been solved by NMR spectroscopy. IN1-55 is dimeric, and each monomer comprises four helices with the zinc tetrahedrally coordinated to His 12, His 16, Cys 40 and Cys 43. IN1-55 exists in two interconverting conformational states that differ with regard to the coordination of the two histidine side chains to zinc. The different histidine arrangements are associated with large conformational differences in the polypeptide backbone (residues 9-18) around the coordinating histidines. The dimer interface is predominantly hydrophobic and is formed by the packing of the N-terminal end of helix 1, and helices 3 and 4. The monomer fold is remarkably similar to that of a number of helical DNA binding proteins containing a helix-turn-helix (HTH) motif with helices 2 and 3 of IN1-55 corresponding to the HTH motif. In contrast to the DNA binding proteins where the second helix of the HTH motif is employed for DNA recognition, IN1-55 uses this helix for dimerization.

PubMed: 9228950
DOI: 10.1038/nsb0797-567

実験手法

SOLUTION NMR