1JPW

Crystal Structure of a Human Tcf-4 / beta-Catenin Complex

Summary for 1JPW

• Entry DOI: 10.2210/pdb1jpw/pdb
• Related: 1G3J 1I7W 1I7X 2bct 3bct
• Descriptor: BETA-CATENIN, transcription factor 7-like 2 (3 entities in total)
• Functional Keywords: beta-catenin, tcf, tcf4, colon cancer, armadillo repeat, transcription factor, cell adhesion
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm : P35222; Nucleus, PML body : Q9NQB0
• Total number of polymer chains: 6
• Total formula weight: 192511.55

Authors

Poy, F.,Lepourcelet, M.,Shivdasani, R.A.,Eck, M.J. (deposition date: 2001-08-03, release date: 2001-12-05, Last modification date: 2023-08-16)

Primary citation

Poy, F.,Lepourcelet, M.,Shivdasani, R.A.,Eck, M.J.
Structure of a human Tcf4-beta-catenin complex.
Nat.Struct.Biol., 8:1053-1057, 2001

PubMed Abstract: The multifunctional protein beta-catenin is important for cell adhesion, because it binds cadherins, and the Wnt signal transduction pathway, where it interacts with the Adenomatous polyposis coli (APC) protein and TCF/Lef family transcription factors. Mutations in APC or in beta-catenin are estimated to trigger formation of over 90% of all colon cancers. In colonic epithelia, these mutations produce elevated levels of Tcf4-beta-catenin, which stimulates a transcriptional response that initiates polyp formation and eventually malignant growth. Thus, disruption of the Tcf4-beta-catenin interaction may be an attractive goal for therapeutic intervention. Here we describe the crystal structure of a human Tcf4-beta-catenin complex and compare it with recent structures of beta-catenin in complex with Xenopus Tcf3 (XTcf3) and mammalian E-cadherin. The structure reveals anticipated similarities with the closely related XTcf3 complex but unexpectedly lacks one component observed in the XTcf3 structure.

PubMed: 11713476
DOI: 10.1038/nsb720

Experimental method

X-RAY DIFFRACTION (2.5 Å)